| Serine/threonine protein phosphatase [Q4QFG0] | |
|---|---|
| Systematic Name | LmjF.15.0220 [Leishmania major] |
| Gene Name | LMJF_15_0220 |
| Molecular Weight | 42443 Da |
| Protein Sequence Size | 374 |
| Function | |
| Charge | -4 |
| Isoelectric Point | 5.8 pH |
| Description | Serine/threonine protein phosphatase (EC 3.1.3.16). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 3.1.3.16 |
| Sequence | >tr|Q4QFG0|Q4QFG0_LEIMA Serine/threonine protein phosphatase (EC 3.1.3.16) - Leishmania major. AHTKRGRFTVDLSVCALSSLPTDTLSVEKENDVLYRLLELLPERYTSKVLEEQRIPEGLI VQILIKARAIIASEPMLVELDSPVYACGDLHGQYYDLVNIFKRCPPLGGTVFGTDAKKAR KETSSSIDDKTYSFLFLGDYVDRGARSVEVVVTLLALKIISPRHMTMLRGNHEDEQIMLL YGFYDECKRRYDIKLFKMFTDLFRILPVAALVNGSIFCVHGGLSAELRFVRDIPDTRPCN VPHSGIICDLLWADPETDLPAGVDWAPSSRRISSVFSERALERFLVENDIDLVCRAHQVV EEGFQFFPDHKKRHLLTIFSATNYCNEFGNRGGILRVDEKGVCSIITLEPPDFVQQRDIM LFRDPLPNPLSHD |
| DNA Sequence | >LmjF15.0220 |||protein phosphatase 1 catalitic subunit, putative|Leishmania major|chr 15|||Manual ATGGCGCACA CGAAGCGAGG GAGATTCACC GTGGATCTGT CCGTGTGTGC GCTCAGTAGCCTTCCTACAG ATACTCTCTC TGTGGAAAAA GAGAATGATG TGCTTTACCG CCTTTTAGAACTTCTTCCTG AGCGCTACAC GAGCAAAGTT TTGGAAGAGC AGCGAATCCC CGAGGGTCTCATCGTGCAAA TTCTCATCAA GGCACGCGCT ATAATAGCCA GTGAGCCGAT GCTTGTCGAATTAGATTCCC CTGTTTACGC CTGCGGTGAT CTACACGGGC AGTACTACGA CTTGGTTAACATTTTCAAGC GGTGCCCACC ACTGGGAGGC ACCGTTTTCG GAACTGATGC GAAGAAGGCACGTAAAGAGA CCAGCTCATC GATCGATGAT AAAACATACA GTTTTCTCTT CCTTGGCGATTATGTCGACC GCGGCGCTCG CTCCGTTGAG GTCGTTGTGA CACTGCTCGC TCTCAAGATTATTTCTCCGC GGCACATGAC TATGCTTCGC GGAAACCACG AGGACGAACA AATCATGCTCTTGTATGGGT TTTACGATGA ATGCAAGAGG CGCTATGACA TCAAACTTTT CAAAATGTTCACCGATTTGT TCCGTATTCT ACCTGTGGCG GCCCTGGTCA ACGGCTCCAT TTTCTGCGTACACGGCGGTC TCTCCGCGGA GCTCCGCTTC GTGCGAGACA TCCCGGACAC GCGTCCGTGCAACGTCCCGC ACTCCGGCAT CATTTGTGAC CTTCTGTGGG CAGACCCCGA AACGGATCTGCCAGCTGGTG TGGACTGGGC CCCGAGCTCG CGCAGAATTT CATCTGTGTT TTCCGAACGGGCACTGGAGC GGTTTTTAGT TGAGAATGAC ATTGATTTGG TGTGCCGCGC ACATCAGGTGGTCGAAGAAG GATTCCAGTT TTTCCCGGAT CACAAAAAAC GGCATTTGCT CACCATCTTCTCGGCAACTA ACTACTGCAA CGAGTTCGGG AATAGAGGCG GCATTTTACG TGTGGATGAGAAGGGTGTAT GCAGCATCAT CACGTTGGAG CCACCAGACT TTGTCCAGCA GCGGGATATCATGCTTTTTC GGGATCCGCT TCCAAACCCT CTGAGCCACG ACTGA |
| Serine/threonine protein phosphatase Q4QFG0] | |
|---|---|
| Metabolite Information | |
| Molecular Function | hydrolase activity |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | protein phosphatase 1, catalytic subunit, alpha isoform 3 [Homo sapiens] | 46 | 2e-72 | 270 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YER133w serthr phosphoprotein phosphatase 1, catalytic chain | Saccharomyces cerevisiae | 45% | 2e-73 | 270 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00115 | Serine/threonine specific protein phosphatases signature | 169-174; | PS00125 | |
| Acylation | N-myristoylation site | 110-115; 215-220; 222-227; 330-335; | PS00008 | |
| Glycosylation | N-glycosylation site | 214-217; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 121-124; 271-274; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 126-129; 127-130; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 4-6; 47-49; 162-164; 269-271; 270-272; 278-280; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 30-36; | PS00007 | |
| Serine/threonine protein phosphatase [Q4QFG0] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1fjmB | |
| Percent Identity | 46% | |
| Target Region | 30-353 | |
| Template Region | 8-288 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 114 | Sidechain |
| HIS | 144 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 60-24-2 | BETA-MERCAPTOETHANOL | 78.133 | C2 H6 O S | OCCS | 1fjm |
| 16397-91-4 | MANGANESE (II) ION | 54.938 | Mn | [Mn+2] | 1fjm |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1697219/Q4QFG0.pdb 2.0 323 = residues | | = | *| Ramachandran plot: 88.8% core 7.4% allow 3.2% gener 0.7% = disall | | = | *| All Ramachandrans: 17 labelled residues (out of 321) = | +| Chi1-chi2 plots: 3 labelled residues (out of 203) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.4 Bad contacts: = 7 | *| Bond len/angle: 5.8 Morris et al class: 1 = 1 2 | +| 3 cis-peptides = | | G-factors Dihedrals: -0.04 Covalent: -0.24 Overall: = -0.11 | | = | | M/c bond lengths: 98.7% within limits 1.3% highlighted = | | M/c bond angles: 91.4% within limits 8.6% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |