| Gamma-tubulin [Q4Q9Z8] | |
|---|---|
| Systematic Name | LmjF.25.0960 [Leishmania major] |
| Gene Name | TUBG |
| Molecular Weight | 50031 Da |
| Protein Sequence Size | 447 |
| Function | Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly |
| Charge | -1 |
| Isoelectric Point | 6.3931 pH |
| Description | Gamma-tubulin. |
| Subcellular Location | basal body; cell soma; microtubule; microtubule organizing center; protein complex; spindle pole; subpellicular microtubul[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4Q9Z8|Q4Q9Z8_LEIMA Gamma-tubulin - Leishmania major PREIITLQAGQCGNQVGSEFWRQLCLEHGIRLDGVVEPYAVGGEDRKDVFFYQADDDHYV PRALLVDLEPRVINAVQRGSMQKLFNSENIFIHKEGGGAGNNWSHGYELGDQVQETLFDM IEREAENSDSLEGFVLTHSIAGGTGSGMGSYLLENLNDKFPKKLIQTYSVFPNQSRGGES DVIVQPYNSLLAVKRLTLHADCVVVLDNTALNRIVTDNLHIASPTVEQMNGLVSTVMAAS TATLRYPGYMNNDLMSMLASLIPTPRCHFVCTGYTPTTLDTSNIQSSVRKTSVHDVMRRL LMPKNMMVSTSMKSGCYISLLNLIQGDVDPAQVHRSLERIRERSPTFIPWGPASIQVILS KKSPYVDTRHRVSGLVMANHTSIHTLFHRTLKQFDMLFGRGVFLDQYRKYGPTKDSLDEF SDARDVVESLVAEYKACESSDYIRNF |
| DNA Sequence | >LmjF25.0960 |||gamma-tubulin|Leishmania major|chr 25|||Manual ATGCCTCGTG AGATCATCAC CCTTCAAGCC GGCCAGTGCG GCAACCAGGT AGGCAGTGAGTTCTGGCGGC AGCTCTGCCT CGAGCACGGC ATCCGACTCG ACGGCGTCGT GGAGCCGTACGCCGTCGGCG GTGAGGATCG CAAAGATGTT TTTTTCTATC AAGCCGACGA CGACCACTACGTGCCGCGTG CGCTGCTCGT GGACCTTGAG CCGCGCGTGA TCAACGCGGT GCAGCGCGGCTCGATGCAGA AGCTCTTCAA CAGCGAGAAC ATCTTCATTC ACAAGGAAGG CGGCGGTGCCGGCAACAACT GGTCTCACGG CTACGAGCTG GGCGACCAGG TGCAGGAGAC GCTCTTCGACATGATCGAGC GAGAGGCCGA GAACAGCGAC AGCCTCGAGG GATTCGTTCT TACTCACTCCATTGCAGGTG GCACGGGAAG CGGCATGGGC AGCTACCTGC TCGAGAACTT GAACGATAAGTTCCCGAAGA AGCTCATCCA GACGTACAGC GTCTTCCCGA ACCAGTCGCG CGGCGGCGAGAGCGACGTCA TTGTCCAGCC GTACAACAGC CTACTAGCCG TGAAGCGACT GACGCTACATGCCGACTGCG TCGTCGTCCT CGACAACACC GCGCTGAACC GTATCGTCAC CGACAACCTCCACATCGCCT CCCCCACGGT GGAGCAGATG AACGGGCTCG TCAGCACCGT CATGGCCGCCTCGACGGCGA CGCTGCGCTA CCCGGGCTAC ATGAACAACG ACTTGATGAG CATGCTAGCCTCGCTCATCC CGACGCCGCG GTGCCACTTT GTGTGCACCG GGTACACCCC GACCACCCTCGACACCTCCA ACATCCAGTC CTCGGTGCGC AAGACGTCCG TGCACGACGT GATGCGTCGACTGCTGATGC CGAAGAACAT GATGGTGTCG ACATCCATGA AGAGTGGCTG CTACATCTCTCTCCTAAACC TCATCCAGGG TGACGTCGAC CCAGCACAGG TGCACCGCTC CCTCGAGCGGATTCGCGAGC GGTCACCGAC GTTCATTCCG TGGGGACCGG CCTCCATCCA GGTCATTCTGTCAAAGAAGT CGCCGTACGT GGACACGCGG CACCGGGTGA GCGGCCTCGT GATGGCAAACCACACGAGCA TCCACACCCT CTTCCACCGC ACGCTGAAGC AGTTTGACAT GCTGTTCGGCCGCGGCGTCT TCCTCGACCA GTACCGAAAG TATGGGCCGA CCAAGGACAG CCTGGACGAGTTCAGCGACG CGAGGGACGT CGTGGAGAGC TTGGTGGCGG AGTACAAGGC GTGTGAGAGCTCCGACTACA TCCGCAACTT CTGA |
| Gamma-tubulin Q4Q9Z8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | GTP binding; GTPase activity; microtubule binding; structural molecule activity |
| Biochemical Pathway | cell growth; microtubule nucleation; microtubule-based movement; protein polymerization |
| Regulatory Pathway | |
| KEGG Pathways | K10389 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | tubulin, gamma 1 [Homo sapiens] | 65 | 1e-171 | 599 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YLR212c gamma tubulin | Saccharomyces cerevisiae | 36% | 3e-88 | 319 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00199 | Tubulin subunits alpha, beta, and gamma signature | 142-148; | PS00227 | |
| Acylation | N-myristoylation site | 11-16; 14-19; 97-102; 98-103; 99-104; 101-106; 143-148; 144-149; 146-151; 232-237; 316-321; 375-380; | PS00008 | |
| Glycosylation | N-glycosylation site | 103-106; 174-177; 380-383; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 195-198; 290-293; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 117-120; 278-281; 293-296; 417-420; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 244-246; 265-267; 288-290; 312-314; 361-363; 391-393; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 436-443; | PS00007 | |
| Gamma-tubulin [Q4Q9Z8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1z5wA | |
| Percent Identity | 54% | |
| Target Region | 1-447 | |
| Template Region | 2-409 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 86-01-1 | GUANOSINE-5'-TRIPHOSPHATE | 523.18 | C10 H16 N5 O14 P3 | O=P(O)(O)OP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 1z5w |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 1z5w |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/830580/Q4Q9Z8.pdb 2.0 447 = residues | | = | *| Ramachandran plot: 87.8% core 9.6% allow 1.5% gener 1.0% = disall | | = | *| All Ramachandrans: 22 labelled residues (out of 445) = | +| Chi1-chi2 plots: 3 labelled residues (out of 265) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 18.7 Bad contacts: = 13 | *| Bond len/angle: 11.9 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.02 Covalent: -0.32 Overall: = -0.11 | | = | *| M/c bond lengths: 98.4% within limits 1.6% highlighted 2 off = graph | *| M/c bond angles: 94.0% within limits 6.0% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |